PrimerSuite

 

Type or paste (Ctrl-V) sequence(s) of the templates here in FASTA format here

Or upload a fasta file: 

Or download a copy of sample sequences here and upload the file for primer design

Bisulfite	Genomic
Repbase check	Report Best primers only
PRIMER DESIGN PARAMETERS
	FORWARD	REVERSE
CpG's in Oligo
Location of CpGs
Fusion sequences
	MINIMIUM	MAXIMIUM
GC content (%) (gDNA only)
Amplicon Size (bp)
Oligo melting temp (°C)
Min Dimer Score Cutoff  ✪
Sodium Concentration (mM)
Min CpGs in amplicon (bisulfite-only)
Primer Score (bisulfite-only)
homopolymer(s)