PrimerSuite

 

Type or paste (Ctrl-V) sequence(s) of the templates here in FASTA format here questionMark

 

Or download a copy of sample sequences here
and upload the file for primer design

 questionMark  questionMark
Repbase check questionMark Report Best primers only questionMark
 
PRIMER DESIGN PARAMETERS
FORWARD REVERSE
 
CpG's in Oligo questionMark
 
Location of CpGs questionMark
 
Fusion sequences  questionMark
 
MINIMIUM MAXIMIUM
 
GC content (%) (gDNA only) questionMark
 
Amplicon Size (bp) questionMark
 
 
 
Oligo melting temp (°C) questionMark
 
Min Dimer Score Cutoff questionMark
 
Sodium Concentration (mM) questionMark
 
Min CpGs in amplicon (bisulfite-only) questionMark
 
Primer Score (bisulfite-only) questionMark
 
homopolymer(s) questionMark