PrimerSuite

 

Type or paste (Ctrl-V) sequence(s) of the templates here in FASTA format here

 

Or download a copy of sample sequences here
and upload the file for primer design

Repbase check Report Best primers only
 
PRIMER DESIGN PARAMETERS
FORWARD REVERSE
 
CpG's in Oligo
 
Location of CpGs
 
Fusion sequences
 
MINIMIUM MAXIMIUM
 
GC content (%) (gDNA only)
 
Amplicon size
 
 
 
Oligo melting temp (°C)
 
ΔG (kcal/mol)
 
Sodium Concentration (mM)
 
Min CpGs in amplicon (bisulfite-only)
 
Primer Score (bisulfite-only)
 
homopolymer(s)